【MIT】分子生物学Part 3：RNA加工与翻译
edx
MITx • 7.28.3x
主讲：
Stephen P. Bell
研究方向：复制和维持动物细胞染色体的细胞机制（研究复制体组装及调节）
2017美国国家科学院院士
Tania A. Baker
研究方向：细胞应激反应中酶催化蛋白质展开、atp依赖性蛋白质降解和蛋白质组重塑的机制和调控
2007美国国家科学院院士
2005美国艺术与科学院院士
视频选集
（14/115）
1.1.Introduction
05:25
1.2.Reading the mRNA in Translation
03:05
1.3.Bacterial mRNAs
08:36
1.4.Eukaryotic mRNAs
06:57
1.5.Structural Properties of tRNAs
09:12
1.6.The Genetic Code
05:40
1.7.0.Wobble Base Pairing
05:58
1.7.5.Segment 7.5 Inosine
01:12
1.8.Specificity of Translation
04:39
1.9.Aminoacyl-tRNA Synthetases
09:02
1.10.Aminoacyl-tRNA Synthetase Proofreading
06:42
1.11.Bacterial Ribosome Structure Overview
04:30
1.12.Bacterial Crystal Structure of the Ribosome
03:38
1.13.Key Functional Regions in the Ribosome Structure
06:04
1.14.Bacterial versus Eukaryotic Ribosomes
05:45
2.1.Phases of Translation
05:07
2.2.GTP-Regulated Auxiliary Translation Factors
04:28
2.3.Structural Characteristics of EF-Tu
04:38
2.4.EF-Tu and Accommodation
06:06
2.5.Fidelity of Correct Codon-Anticodon Pairing
06:27
2.6.Mechanism of Peptide Bond Formation
05:47
2.7.Translocation
10:04
2.8.Energetics and EF-G
09:50
3.1.Goals of Initiation
03:00
3.2.Bacterial Initiation Factors and Initiator tRNA
08:36
3.3.Bacterial Initiation Continued
04:38
3.4.Experimental Ordering of Initiation Events
04:03
3.5.Eukaryotic Initiation, Part I
08:17
3.6.Eukaryotic Initiation, Part II
05:46
3.7.Eukaryotic Initiation, Part III
04:44
3.8.Eukaryotic Initiation and Factor Comparison
04:50
3.9.Internal Ribosome Entry Sites (IRESs)
06:28
3.10.CrPV Example of an IRES
06:51
3.11.More IRES Examples
07:52
3.12.Termination
09:35
3.13.Bacterial Translation Termination and Ribosome Recycling
08:50
3.14.Eukaryotic Translation Termination and Ribosome Recycling
06:32
3.15.Toeprinting Assay
04:05
3.16.Hydroxyl Radical Footprinting
10:49
3.17.Antibiotics and Analogs
06:59
4.1.Amino Acid Incorporation Assay
05:02
4.2.Polysome Profiling
04:33
4.3.Ribo-seq
04:01
4.4.Translational Regulation
08:12
4.5.Examples of Assaying for Translation Regulation
08:14
4.6.Rate-Limiting Step
05:28
4.7.Bacterial Translational Regulation, Part 1
04:09
4.8.Bacterial Translational Regulation, Part 2
06:24
4.9.Bacterial Translational Regulation, Part 3
07:59
4.10.Bacterial Translational Regulation, Part 4
05:34
4.11.Eukaryotic Translational Regulation, Part 1
07:52
4.12.Eukaryotic Translational Regulation, Part 2
06:18
4.13.Eukaryotic Translational Regulation, Part 3
03:15
4.14.Upstream ORFs, Part 1
05:34
4.15.Upstream ORFs, Part 2
07:44
4.16.uORFs in GCN4
14:19
4.17.Translation Optimization
04:14
5.1.Introduction to RNA Splicing
03:07
5.2.Discovery of Splicing and R-Loop Mapping
06:37
5.3.Types of Splicing Reactions
02:20
5.4.Self-Splicing Introns, Mechanistic Overview
07:16
5.5.Self-Splicing Introns, Detailed Structure
04:58
5.6.Assays for RNA Structure, RNase Digestion
04:29
5.7.Assays for RNA Structure, DMS-seq and SHAPE-seq
09:16
5.8.Assays for RNA Structure, Computational Prediction
03:53
5.9.Splicing Mechanism
09:49
5.10.Discovery of Splicing Mechanism, Cis Components
10:39
5.11.Discovery of Splicing Mechanism, Trans Components
04:22
5.12.Spliceosome Cycle Part 1
07:18
5.13.Spliceosome Cycle Part 2
05:31
5.14.Spliceosome Cycle Part 3
07:51
6.1.Splicing Proofreading, Introduction
05:08
6.2.Splicing Proofreading, Prp5 and Prp16
04:38
6.3.Splicing Proofreading, ATP-Dependent Reactions
09:37
6.4.Alternative Splicing, Part 1
07:37
6.5.Alternative Splicing, Part 2
07:11
6.6.Assays for Alternative Splicing, RT-PCR
05:00
6.7.Assays for Alternative Splicing, RNA-seq
04:34
6.8.Importance of Alternative Splicing in Metazoans
03:25
6.9.Mechanisms of Alternative Splicing
03:34
6.10.Alternative Splicing Examples
09:18
6.11.Genomic Introns and Exons
03:13
6.12.Defining Splice Sites
03:47
6.13.Regulation of Splicing, Enhancers and Repressors
06:58
6.14.Effect of Chromatin on Splicing
03:08
6.15.Assays for Regulation of Splicing
08:23
6.16.Examples of Regulation of Splicing
09:06
6.17.Conclusion
03:50
7.1.Introduction to RNA Turnover
01:26
7.2.RNA Decay
07:27
7.3.RNA Half-Lives
06:32
7.4.Ribonucleases and In Vitro Nuclease Assay
11:19
7.5.In Vivo Half-Life Assay
04:07
7.6.RNA Pulse-Chase Assay
05:36
7.7.Protection from Bacterial mRNA Decay
04:06
7.8.Bacterial RNA Degradosome
04:48
7.9.Bacterial mRNA Decay Mechanisms
09:46
7.10.Bacterial Decay of RNA with Secondary Structure
03:29
7.11.Bacterial Rescue of Stalled Ribosomes
04:25
7.12.Eukaryotic mRNA Decay
07:40
7.13.Eukaryotic Deadenylases
02:04
7.14.Measuring polyA Tail Length
04:21
7.15.Eukaryotic RNA Decapping
05:52
7.16.Eukaryotic Ribosome Synthesis and Decay
08:43
7.17.Eukaryotic tRNA Decay
01:02
8.1.Ribosomes as Detectors
03:09
8.2.Trp Attenuator
10:48
8.3.Eukaryotic Translation-Dependent mRNA Surveillance
03:57
8.4.Discovery of Nonsense-Mediated Decay, Part 1
09:45
8.5.Discovery of Nonsense-Mediated Decay, Part 2
07:38
8.6.Nonsense-Mediated Decay, mRNA Recognition
07:55
8.7.Nonsense-Mediated Decay, Vertebrate mRNA decay and Ribosome Recycling
07:43
8.8.Nonsense-Mediated Decay, Yeast and Fly mRNA Decay and Ribosome Recycling
03:43
8.9.Nonstop-Mediated versus No-go Decay (mRNA Recognition)
08:42
8.10.Nonstop-Mediated versus No-go Decay (mRNA Decay, Ribosome Recycling, Peptid
10:36
研途大咖带学带刷带押，开学直接起飞
现在上车还来得及！
顶部