PLACENTA – MOVIE 1: 2D going through: w19 placenta optical layers; vessels and cytotrophoblast layer.
The placenta is a large organ in the woman’s uterus during pregnancy, which provides oxygen and nutrients to the embryo/foetus and removes waste products from the baby’s blood. These videos reveal some details about the fine structure of human placenta in three-dimension, using the cytotrophoblast marker PEG10 as well as the immunomarker CD34, which depicts the blood vessels inside the organ.
METHOD: iDISCO+ volume immunostaining and clearing
TISSUE: Human placenta from abortion, w19.
ANTIBODIES: PEG10 (green); CD34 (red)
MOVIE DESCRIPTION: 2D going through: w19 placenta as serial of optical slices. PEG10 (green in the movies) is mainly the marker of cytotrophoblast. CD34 (red in the movies) depicts the endothelium of blood vessels.
FURTHER INFORMATION AND OTHER MOVIES: www.volume-imaging.com
