FlowRACS is a high-throughput flow cytometer featuring metabolic phenotype-based cell characterization and sorting with single-cell Raman spectroscopy. Inside FlowRACS, cells are analyzed and sorted without labeling or staining. Using a patented technology, cells are aligned and passed sequentially through a checkpoint, where Raman spectrum of each cell is accurately determined. Live cells with targeted Raman spectra or metabolic functions can then be collected for desired downstream analysis.